ROLE OF SUGAR CHAINS IN THE INVITRO ACTIVITY OF RECOMBINANT HUMAN INTERLEUKIN-5

被引：35

作者：

KODAMA, S ^{[1
]}

TSUJIMOTO, M ^{[1
]}

TSURUOKA, N ^{[1
]}

SUGO, T ^{[1
]}

ENDO, T ^{[1
]}

KOBATA, A ^{[1
]}

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT BIOCHEM,TOKYO 113,JAPAN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 211卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb17624.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structures of 0-linked sugar chains of recombinant human interleukin 5 (rhIL-5) produced by Chinese hamster ovary cells were determined employing high-performance anion-exchange column chromatography under high pH conditions. The core sequence was elucidated to be Galbeta1-->3-GalNAc by its susceptibility to endo-alpha-N-acetylgalactosaminidase and sialic acid linkages were determined using sialidases of different specificities. To investigate the role of sugar chains in the in-vitro activity of rhIL-5, it was digested with various glycosidases. While removal of N-linked sugar chains resulted in 2.8-fold increase of the activity, de-0-glycosylated rhIL-5 showed 10-fold higher activity than intact rhIL-5, suggesting that the presence of 0-linked sugar chains suppresses the activity more effectively than that of N-linked chains. While incubation of de-N-glycosylated rhIL-5 at 70-degrees-C for 30 min decreased the activity, intact and de-0-glycosylated rhIL-5 lost little activity, suggesting that N-linked sugar chains contributed to the thermostability of the molecule.

引用

页码：903 / 908

页数：6

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