FLUORESCENCE DETECTION OF ENZYMATICALLY FORMED HYDROGEN-PEROXIDE IN AQUEOUS-SOLUTION AND IN REVERSED MICELLES

被引：43

作者：

FERRER, AS ^{[1
]}

SANTEMA, JS ^{[1
]}

HILHORST, R ^{[1
]}

VISSER, AJWG ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN, DEPT BIOCHEM, DREIJENLAAN 3, 6703 HA WAGENINGEN, NETHERLANDS

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 187卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90429-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive enzymatic assay for oxidase reactions both in aqueous solution and in hexadecyltrimethylam-moniumbromide (CTAB) reversed micelles has been developed. The assay is based on the fluorescence detection of dichlorofluorescein, which is formed by hydrogen peroxide oxidation of the nonfluorescent precursor dichlorofluorescin. Hydrogen peroxide as product of the reaction catalyzed by glucose oxidase served to select the reaction conditions. The reaction rate is distinctly enhanced in CTAB reversed micelles as compared to the rate in aqueous solution. This effect, combined with the high sensitivity owing to the strong fluorescence of dichlorofluorescein, makes the assay attractive for the detection of low enzyme, substrate, or peroxide concentrations. © 1990.

引用

页码：129 / 132

页数：4

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