IMPORTANCE OF THE G27-A43 MISMATCH AT THE ANTICODON STEM OF ESCHERICHIA-COLI TRANSFER-RNA THR2

被引：7

作者：

KOMINE, Y ^{[1
]}

INOKUCHI, H ^{[1
]}

机构：

[1] KYOTO UNIV,FAC SCI,DEPT BIOPHYS,KYOTO 606,JAPAN

来源：

FEBS LETTERS | 1990年 / 272卷 / 1-2期

关键词：

Amber suppressor; E; coli; G27-A43; mismatch; tRNA[!sup]Thr[!/sup;

D O I：

10.1016/0014-5793(90)80447-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tRNAThr isoacceptor of E. coli has a G-A mismatch at positions 27-43. When the anticodon of this tRNA was converted to an amber anticodon (CUA), this tRNA showed suppressor activity in E. coli. Moreover, introduction of the base pair (G-C or U-A) at positions 27-43 of this suppressor tRNA reduced its suppressor activity. These results indicate that the G27-A43 mismatch is necessary for full function of tRNAThr. © 1990.

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页码：55 / 57

页数：3

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