A MICROSCOPE STAGE TEMPERATURE CONTROLLER FOR THE STUDY OF WHOLE-CELL OR SINGLE-CHANNEL CURRENTS

被引：15

作者：

CHABALA, LD ^{[1
]}

SHERIDAN, RE ^{[1
]}

HODGE, DC ^{[1
]}

POWER, JN ^{[1
]}

WALSH, MP ^{[1
]}

机构：

[1] CALTECH, DIV BIOL, PASADENA, CA 91125 USA

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1985年 / 404卷 / 04期

关键词：

D O I：

10.1007/BF00585351

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The construction of a microscope stage temperature controller is described that works equally well with upright or inverted microscopes. The control circuit directly regulates the bath temperature near the physiological preparation from 0.0.degree. C to 40.0.degree. C with a stability of .+-. 0.1.degree. C; it allows biophysical studies of whole-cell or single channel currents to be carried out at different temperatures without introducing additional electrical noise into the measurements. The device may also be of interest to neurobiologists who work with tissue-culture preparations.

引用

页码：374 / 377

页数：4

共 6 条

[1]

CHABALA LD, 1985, BIOPHYS J

[2]

Corey, 1983, SINGLE CHANNEL RECOR, P53

[3]

GURNEY AM, 1985, J GEN PHYSL

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HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[5]

Neher E, 1982, TECHNIQUES CELLULAR, P1

[6] A TRANSITION STATE THEORY APPROACH TO KINETICS OF CONDUCTANCE CHANGES IN EXCITABLE MEMBRANES [J].

TSIEN, RW ;

NOBLE, D .

JOURNAL OF MEMBRANE BIOLOGY, 1969, 1 (03) :248-&

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