ROLE OF 2-KETOBUTYRATE AS AN ALARMONE IN ESCHERICHIA-COLI-K12 - INHIBITION OF ADENYLATE-CYCLASE ACTIVITY MEDIATED BY THE PHOSPHOENOLPYRUVATE - GLYCOSE PHOSPHOTRANSFERASE TRANSPORT-SYSTEM

被引：14

作者：

DANIEL, J

JOSEPH, E

DANCHIN, A

机构：

来源：

MOLECULAR AND GENERAL GENETICS | 1984年 / 193卷 / 03期

关键词：

D O I：

10.1007/BF00382085

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

2-Ketobutyrate and its analogs strongly and transiently inhibited the rate of .beta.-galactosidase synthesis in E. coli K12. This effect was ascribed to a strong and transient inhibition of adenylate cyclase activity. Using pts mutants, it was found that the likely target of 2-ketobutyrate and its analogs is the phosphoenolpyruvate:glycose phosphotransferase transport system. These results support the proposal tht 2-ketobutyrate, a precursor of isoleucine, acts as an E. coli alarmone monitoring the passage from anaerobic to aerobic growth conditions.

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页码：467 / 472

页数：6

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