MOLECULAR-CLONING OF 3 CDNAS THAT ENCODE CYSTEINE PROTEINASES IN THE DIGESTIVE GLAND OF THE AMERICAN LOBSTER (HOMARUS-AMERICANUS)

被引：53

作者：

LAYCOCK, MV

MACKAY, RM

DIFRUSCIO, M

GALLANT, JW

机构：

[1] Institute for Marine Biosciences, National Research Council of Canada, Halifax, NS B3H 3Z1

来源：

FEBS LETTERS | 1991年 / 292卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

CYSTEINE PROTEINASE; CDNA CLONING; AMINO ACID SEQUENCE; LOBSTER;

D O I：

10.1016/0014-5793(91)80847-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three clones were isolated from a lobster digestive gland cDNA library, using oligonucleotide probes based on the partial amino terminal sequence of a digestive cysteine proteinase. The cDNAs, LCP1, LCP2 and LCP3 encode preproenzymes of 322, 323 and 321 amino acid residues, and putative mature enzymes of 217, 216 and 215 residues, respectively. Calculated mature protein molecular masses are 23386 (LCP1), 23093 (LCP2) and 23255 (LCP3) Sequence alignments show that the lobster enzymes are more similar to L (55-62% identity) than H (42-44%) or B (22-24%) cathepsins. Southern analysis indicated as many as eleven genes related to the three cDNAs.

引用

页码：115 / 120

页数：6

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