LIPASE CATALYSIS IN ORGANIC-SOLVENTS

被引：11

作者：

CAO, SG

FENG, Y

LIU, ZB

DING, ZT

CHENG, YH

机构：

[1] National Laboratory of Enzyme Engineering, Jilin University, Changchun

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 32卷

关键词：

LIPASE; ESTERIFICATION; TRANSESTERIFICATION; BIOCATALYSIS; ORGANIC MEDIA;

D O I：

10.1007/BF02922144

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conditions for esterification and transesterification catalyzed by porcine pancreatic lipase in organic media were studied. It was found that the enzyme reaction was dependent on the following factors: the pH at which the enzyme powder was prepared from its solution, the polarity of organic media, the reaction temperature, the water content in reaction system, and the substrate structures. Effects of the above factors on enzyme activity were discussed.

引用

页码：7 / 13

页数：7

共 7 条

[1] COLJA L, 1987, BIOTECHNOL BIOENG, V30, P81
[2] Desnuelle P, 1971, BIOCHEMISTRY-US, V10, P2143
[3] ENZYMATIC CATALYSIS IN MONOPHASIC ORGANIC-SOLVENTS
DORDICK, JS
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (04) : 194 - 211
[4] INADA Y, 1990, BIOCATALYSIS, V3, P317
[5] KLIBANOV A M, 1986, Chemtech, V16, P354
[6] ENZYMATIC CATALYSIS IN ANHYDROUS ORGANIC-SOLVENTS
KLIBANOV, AM
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (04) : 141 - 144
[7] WHY DOES RIBONUCLEASE IRREVERSIBLY INACTIVATE AT HIGH-TEMPERATURES
ZALE, SE
KLIBANOV, AM
[J]. BIOCHEMISTRY, 1986, 25 (19) : 5432 - 5444

← 1 →