IN-VITRO STRUCTURAL AND FUNCTIONAL-RELATIONSHIPS BETWEEN PREOSTEOCLASTIC AND BONE ENDOTHELIAL-CELLS - A JUXTACRINE MODEL FOR MIGRATION AND ADHESION OF OSTEOCLAST PRECURSORS

被引：26

作者：

FORMIGLI, L

ORLANDINI, SZ

BENVENUTI, S

MASI, L

PINTO, A

GATTEI, V

BERNABEI, PA

ROBEY, PG

COLLINOSDOBY, P

BRANDI, ML

机构：

[1] UNIV FLORENCE, SCH MED, DEPT CLIN PHYSIOPATHOL, ENDOCRINE UNIT, I-50139 FLORENCE, ITALY

[2] UNIV FLORENCE, DEPT ANAT, I-50139 FLORENCE, ITALY

[3] UO HEMATOL, I-50139 FLORENCE, ITALY

[4] CTR RIFERIMENTO ONCOL, LEUKEMIA UNIT, I-3308 AVIANO, ITALY

[5] NIDR, BONE RES BRANCH, BETHESDA, MD 20892 USA

[6] WASHINGTON UNIV, DEPT BIOL, ST LOUIS, MO 63130 USA

[7] WASHINGTON UNIV, DIV BONE & MINERAL METAB, ST LOUIS, MO 63130 USA

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1995年 / 162卷 / 02期

关键词：

D O I：

10.1002/jcp.1041620206

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The role of vascularization in the process of bone resorption has not been clarified. The interactions between vascular endothelium and osteoclast progenitors were analyzed using clonal cell lines of bone-derived endothelial and preosteoclastic cells. Insulin-like growth factor I is a major chemotactic stimulator of preosteoclastic cell migration mediated by bone endothelial cells. Osteoclast precursors rapidly adhered to bone endothelial monolayers. This phenomenon appeared to be cell-specific and mediated through the binding of vitronectin and fibronectin receptors to fibronectin. In addition, direct contact with bone endothelial cells induced osteoclast progenitors to differentiate into more mature elements, with the tendency to cluster together to form large multinucleated cells. These findings demonstrated specific in vitro interactions between bone endothelial cells and osteoclast progenitors, offering a new model for understanding the molecular mechanisms which direct the processes of osteoclast recruitment and ontogeny. (C) 1995 Wiley-Liss, Inc.

引用

页码：199 / 212

页数：14

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