CLONING AND EXPRESSION OF GRK5 - A MEMBER OF THE G-PROTEIN-COUPLED RECEPTOR KINASE FAMILY

Guanine nucleotide binding protein (G-protein)-coupled receptor kinases (GRKs) specifically phosphorylate the agonist-occupied form of G-protein-coupled receptors such as the beta2-adrenergic receptor and rhodopsin. The best characterized members of this family include the beta-adrenergic receptor kinase (betaARK) and rhodopsin kinase. To identify additional members of the GRK family, the polymerase chain reaction was used to amplify human heart cDNA using degenerate oligonucleotide primers from highly conserved regions unique to the GRK family. Here we report the isolation of a cDNA that encodes a 590-amino acid protein kinase, termed GRK5, which has 34.8% and 47.2% amino acid identities with betaARK and rhodopsin kinase, respectively. Interestingly, GRK5 has an even higher homology with Drosophila GPRK-2 (71.0% identity) and the recently identified human IT11 (69.1 % identity). Northern blot analysis of GRK5 with selected human tissues reveals a message of almost-equal-to 3 kilobases with highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney. GRK5, overexpressed in Sf9 insect cells using the baculovirus system, was able to phosphorylate rhodopsin in a light-dependent manner. In addition, GRK5 neither contains a consensus sequence for isoprenylation like rhodopsin kinase nor is activated by G-protein betagamma subunits like betaARK1. Thus, GRK5 represents a member of the GRK family that likely has a unique physiological role.