CONVERSION OF L-SORBOSONE TO L-ASCORBIC-ACID BY A NADP-DEPENDENT DEHYDROGENASE IN BEAN AND SPINACH LEAF

被引：47

作者：

LOEWUS, MW ^{[1
]}

BEDGAR, DL ^{[1
]}

SAITO, K ^{[1
]}

LOEWUS, FA ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,INST BIOL CHEM,PULLMAN,WA 99164

来源：

PLANT PHYSIOLOGY | 1990年 / 94卷 / 03期

关键词：

D O I：

10.1104/pp.94.3.1492

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An NADP-dependent dehydrogenase catalyzing the conversion of L-sorbosone to L-ascorbic acid has been isolated from Phaseolus vulgaris L. and Spinacia oleracea L. and partially purified. It is stable at -20°C for up to 8 months. Molecular masses, as determined by gel filtration, were 21 and 29 kilodaltons for bean and spinach enzymes, respectively. Km for sorbosone were 12 ± 2 and 18 ± 2 millimolar and for NADP+, 0.14 ± 0.05 and 1.2 ± 0.5 millimolar, for bean and spinach, respectively. Lycorine, a purported inhibitor of L-ascorbic acid biosynthesis, had no effect on the reaction.

引用

页码：1492 / 1495

页数：4

共 18 条

[1] LYCORINE AS AN INHIBITOR OF ASCORBIC-ACID BIOSYNTHESIS [J].