HOLE, THE GENE CODING FOR THE THETA SUBUNIT OF DNA-POLYMERASE-III OF ESCHERICHIA-COLI - CHARACTERIZATION OF HOLE MUTANT AND COMPARISON WITH A DNAQ (EPSILON-SUBUNIT) MUTANT

被引:61
作者
SLATER, SC
LIFSICS, MR
ODONNELL, M
MAURER, R
机构
[1] CASE WESTERN RESERVE UNIV,SCH MED,DEPT MOLEC BIOL & MICROBIOL,CLEVELAND,OH 44106
[2] CORNELL UNIV,COLL MED,DEPT MICROBIOL,NEW YORK,NY 10021
[3] HOWARD HUGHES MED INST,NEW YORK,NY 10021
关键词
D O I
10.1128/jb.176.3.815-821.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA polymerase III holoenzyme is a multiprotein complex responsible for the bulk of chromosomal replication in Escherichia coli and Salmonella typhimurium. The catalytic core of the holoenzyme is an alpha epsilon theta heterotrimer that incorporates both a polymerase subunit (alpha; dnaE) and a proofreading subunit (epsilon; dnaQ). The role of theta is unknown. Here, we describe a null mutation of holE, the gene for theta. A strain carrying this mutation was fully viable and displayed no mutant phenotype. In contrast, a dnaQ null mutant exhibited poor growth, chronic SOS induction, and an elevated spontaneous mutation rate, like dnaQ null mutants of S. typhimurium described previously. The poor growth was suppressible by a mutation affecting alpha which was identical to a suppressor mutation identified in S. typhimurium. A double mutant null for both holE and dnaQ was indistinguishable from the dnaQ single mutant. These results show that the theta subunit is dispensable in both dnaQ(+) and mutant dnaQ backgrounds, and that the phenotype of E mutants cannot be explained on the basis of interference with theta function.
引用
收藏
页码:815 / 821
页数:7
相关论文
共 43 条
[1]  
Bachmann BJ, 1987, ESCHERICHIA COLI SAL, V2, P1190
[2]   THE ESCHERICHIA-COLI DNA POLYMERASE-III HOLOENZYME CONTAINS BOTH PRODUCTS OF THE DNAX GENE, TAU AND GAMMA, BUT ONLY TAU IS ESSENTIAL [J].
BLINKOVA, A ;
HERVAS, C ;
STUKENBERG, PT ;
ONRUST, R ;
ODONNELL, ME ;
WALKER, JR .
JOURNAL OF BACTERIOLOGY, 1993, 175 (18) :6018-6027
[3]   ALLELIC EXCHANGE IN ESCHERICHIA-COLI USING THE BACILLUS-SUBTILIS SACB GENE AND A TEMPERATURE-SENSITIVE PSC101 REPLICON [J].
BLOMFIELD, IC ;
VAUGHN, V ;
REST, RF ;
EISENSTEIN, BI .
MOLECULAR MICROBIOLOGY, 1991, 5 (06) :1447-1457
[4]   2 PATHWAYS OF DIVISION INHIBITION IN UV-IRRADIATED ESCHERICHIA-COLI [J].
BURTON, P ;
HOLLAND, IB .
MOLECULAR & GENERAL GENETICS, 1983, 190 (01) :128-132
[5]  
Carter Jimmy, COMMUNICATION
[6]   ISOLATION, SEQUENCING AND OVEREXPRESSION OF THE GENE ENCODING THE THETA-SUBUNIT OF DNA POLYMERASE-III HOLOENZYME [J].
CARTER, JR ;
FRANDEN, MA ;
AEBERSOLD, R ;
KIM, DR ;
MCHENRY, CS .
NUCLEIC ACIDS RESEARCH, 1993, 21 (14) :3281-3286
[7]   ONE-STEP PREPARATION OF COMPETENT ESCHERICHIA-COLI - TRANSFORMATION AND STORAGE OF BACTERIAL-CELLS IN THE SAME SOLUTION [J].
CHUNG, CT ;
NIEMELA, SL ;
MILLER, RH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) :2172-2175
[8]   CONTRIBUTION OF 3'-]5' EXONUCLEASE ACTIVITY OF DNA POLYMERASE-III HOLOENZYME FROM ESCHERICHIA-COLI TO SPECIFICITY [J].
FERSHT, AR ;
KNILLJONES, JW .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 165 (04) :669-682
[9]   LEVELS OF EPSILON, AN ESSENTIAL REPLICATION SUBUNIT OF ESCHERICHIA-COLI DNA POLYMERASE-III, ARE CONTROLLED BY HEAT-SHOCK PROTEINS [J].
FOSTER, PL ;
MARINUS, MG .
JOURNAL OF BACTERIOLOGY, 1992, 174 (23) :7509-7516
[10]   PRESENCE OF THE DNAQ-RNH DIVERGENT TRANSCRIPTIONAL UNIT ON A MULTICOPY PLASMID INHIBITS INDUCED MUTAGENESIS IN ESCHERICHIA-COLI [J].
FOSTER, PL ;
SULLIVAN, AD ;
FRANKLIN, SB .
JOURNAL OF BACTERIOLOGY, 1989, 171 (06) :3144-3151