DECREASED PROPORTION OF TYPE-I MYOFIBERS IN SKELETAL-MUSCLE OF DOGS WITH CHRONIC HEART-FAILURE

Background. Whether biochemical and histological abnormalities of skeletal muscle (SM) develop in patients with chronic heart failure (HF) remains controversial. In the present study, dogs with chronic HF were used to examine potential alterations of SM fiber type, fiber size, number of capillaries per fiber (C/F), beta-adrenergic receptor density (B(max)), and fiber ultrastructural integrity. Methods and Results. HF was produced in 17 dogs by sequential intracoronary microembolizations. Biopsies of the lateral head of the triceps muscle were used in all studies. Type I and type II fibers were differentiated by myofibrillar ATPase (Ph 9.4 or 4.2). B(max) was assessed by radioligand binding and SM ultrastructure by transmission electron microscopy. Comparisons were made with biopsies obtained from nine control dogs. The percentage of SM type I fibers was reduced in HF dogs compared with control dogs (19+/-2% versus 32+/-5%) (p<0.001), whereas the percentage of SM type II fibers was increased (81+/-2% versus 68+/-5%) (p<0.001). The change in fiber type composition was not associated with a preferential atrophy or hypertrophy of either fiber type. There was no difference in SM B(max) (198.9+/-14.3 versus 186.8+/-17.3 fmol/mg protein) or in C/F (5.37+/-0.26 versus 5.84+/-0.21) between HF dogs and control dogs. No ultrastructural abnormalities were present in SM fibers of HF dogs. Conclusions. In dogs with HF, there is a decrease in the relative composition of the slow-twitch type I SM fibers and an increase in fast-twitch type II fibers. The shift in fiber type composition is not associated with preferential atrophy of either fiber type or with a reduction in C/F, beta-adrenergic receptor density, or structural abnormalities of the myofibers.