CELL-PROLIFERATION OF HUMAN BLADDER-TUMORS DETERMINED BY BRDURD AND KI-67 IMMUNOSTAINING

被引:65
作者
TSUJIHASHI, H
NAKANISHI, A
MATSUDA, H
UEJIMA, S
KURITA, T
机构
[1] Department of Urology, Kinki University, School of Medicine, Osaka-Sayama Osaka 589
基金
美国国家卫生研究院;
关键词
BLADDER TUMORS; IMMUNOSTAINING;
D O I
10.1016/S0022-5347(17)38475-6
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
The cell proliferation of bladder tumors was assessed in situ using BrdUrd (bromodeoxyuridine) and Ki-67 immunostaining. BrdUrd is incorporated into S-phase cells, and Ki-67 monoclonal antibody recognizes a nuclear antigen present in proliferating, but not resting, cells. The percentage of labeled cells was expressed as the labeling index (LI). The average BrdUrd LI obtained applying this method to normal epithelium and to transitional cell carcinoma were 4.1% and 13.1%, respectively, while the Ki-67 LI had average values of 6.2% and 17.8%, respectively. Labeled cells were distributed throughout both the basal and surface layers of transitional cell carcinoma. In general, the value of the BrdUrd LI was correlated to that of the Ki-67 LI. A relatively large fraction of labeled cells was found in high grade or invasive tumors. Bladder tumors with lymph node involvement had higher LI than those without. In addition, a high frequency of S-phase cells within tumor tissue appeared to indicate a great potential for malignancy and thus a poor prognosis. These findings indicate that immunostaining with BrdUrd and Ki-67 may be useful tools for easy and quick evaluation of proliferating cells in bladder tumors.
引用
收藏
页码:846 / 849
页数:4
相关论文
共 20 条
[1]   THE USE OF THE MONOCLONAL-ANTIBODY KI-67 IN THE IDENTIFICATION OF PROLIFERATING CELLS - APPLICATION TO SURGICAL NEUROPATHOLOGY [J].
BURGER, PC ;
SHIBATA, T ;
KLEIHUES, P .
AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1986, 10 (09) :611-617
[2]  
FARROW GM, 1976, CANCER RES, V36, P2495
[3]  
GERDES J, 1984, J IMMUNOL, V133, P1710
[4]   PRODUCTION OF A MOUSE MONOCLONAL-ANTIBODY REACTIVE WITH A HUMAN NUCLEAR ANTIGEN ASSOCIATED WITH CELL-PROLIFERATION [J].
GERDES, J ;
SCHWAB, U ;
LEMKE, H ;
STEIN, H .
INTERNATIONAL JOURNAL OF CANCER, 1983, 31 (01) :13-20
[6]  
HAINAU B, 1974, CANCER-AM CANCER SOC, V33, P115, DOI 10.1002/1097-0142(197401)33:1<115::AID-CNCR2820330118>3.0.CO
[7]  
2-V
[8]  
HOSHINO T, 1979, CANCER, V44, P956, DOI 10.1002/1097-0142(197909)44:3<956::AID-CNCR2820440325>3.0.CO
[9]  
2-C
[10]   USE OF AVIDIN-BIOTIN-PEROXIDASE COMPLEX (ABC) IN IMMUNOPEROXIDASE TECHNIQUES - A COMPARISON BETWEEN ABC AND UNLABELED ANTIBODY (PAP) PROCEDURES [J].
HSU, SM ;
RAINE, L ;
FANGER, H .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1981, 29 (04) :577-580