AN EFFICIENT OXYGEN INDEPENDENT 2-PHOTON PHOTOSENSITIZATION MECHANISM

被引：34

作者：

SMITH, G

MCGIMPSEY, WG

LYNCH, MC

KOCHEVAR, IE

REDMOND, RW

机构：

[1] HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT DERMATOL,WELLMAN LABS PHOTOMED,BOSTON,MA 02114

[2] WORCESTER POLYTECH INST,DEPT CHEM,WORCESTER,MA 01609

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1994年 / 59卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1994.tb05012.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel oxygen-independent photosensitization mechanism from the upper triplet state (T-n) of rose bengal has been demonstrated by selectively populating T-n by sequential two-color laser excitation. Products formed from T-n inhibit red blood cell acetylcholinesterase and decrease viability of P388D(1) mouse macrophage monocyte cells as measured by trypan blue exclusion assay. Laser flash photolysis studies indicate that T-n reacts efficiently, as evidenced by permanent photobleaching of T-1 absorption, with chemical yields approaching unit efficiency. This mechanism may have application for oxygen deficient photosensitization under high intensity, pulsed laser irradiation.

引用

页码：135 / 139

页数：5

共 10 条

[1] A WAVELENGTH DEPENDENT MECHANISM FOR ROSE BENGAL-SENSITIZED PHOTOINHIBITION OF RED-CELL ACETYLCHOLINESTERASE [J].