BACULOVIRUS EXPRESSION OF NONSTRUCTURAL PROTEIN-NS2 AND CORE PROTEIN-VP7 OF AFRICAN HORSESICKNESS VIRUS SEROTYPE-3 AND THEIR USE AS ANTIGENS IN AN INDIRECT ELISA

被引：11

作者：

BREMER, CW

DUPLESSIS, DH

VANDIJK, AA

机构：

[1] Biochemistry Division, Onderstepoort Veterinary Institute, Onderstepoort, 0110

来源：

JOURNAL OF VIROLOGICAL METHODS | 1994年 / 48卷 / 2-3期

关键词：

AHSV3; BACULOVIRUS; NS2; VP7; INDIRECT ELISA;

D O I：

10.1016/0166-0934(94)90123-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-structural protein NS2 and core protein VP7 of African horsesickness virus serotype 3 (AHSV3) were expressed in Spodoptera frugiperda cells by recombinant baculoviruses containing the relevant genes. These proteins were purified and analysed by polyacrylamide gel electrophoresis and Western blot. NS2 and VP7 were used separately as antigens in an indirect ELISA for the detection of AHSV antibodies. Both antigens cross-reacted with hyperimmune guinea-pig antisera to infected cell lysates of all nine known AHSV serotypes and to antisera obtained from horses immunized with attenuated virus of seven AHSV serotypes.

引用

页码：245 / 256

页数：12

共 35 条

[1] A COMPETITIVE ELISA FOR THE DETECTION OF ANTI-TUBULE ANTIBODIES USING A MONOCLONAL-ANTIBODY AGAINST BLUETONGUE VIRUS NONSTRUCTURAL PROTEIN NS1 [J].