LIPASE-CATALYZED ALCOHOLYSIS OF TRIGLYCERIDES FOR THE PREPARATION OF 2-MONOGLYCERIDES

被引：46

作者：

MILLQVIST, A

ADLERCREUTZ, P

MATTIASSON, B

机构：

[1] Department of Biotechnology, Chemical Center, Lund University

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1994年 / 16卷 / 12期

关键词：

LIPASE; ORGANIC SOLVENTS; ALCOHOLYSIS; HYDROLYSIS; 2-MONOGLYCERIDES; WATER ACTIVITY; OPERATIONAL STABILITY;

D O I：

10.1016/0141-0229(94)90140-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity O.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200-300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions.

引用

页码：1042 / 1047

页数：6

共 21 条

[1] LIPASE G-CATALYZED SYNTHESIS OF MONOGLYCERIDES IN ORGANIC-SOLVENT AND ANALYSIS BY HPLC [J].