CLONING AND SEQUENCING OF THE URA3 LOCUS OF THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA AND ITS USE FOR THE GENERATION OF A DELETION BY GENE REPLACEMENT

被引：38

作者：

MERCKELBACH, A

GODECKE, S

JANOWICZ, ZA

HOLLENBERG, CP

机构：

[1] RHEIN BIOTECH GMBH,D-40595 DUSSELDORF,GERMANY

[2] HEINRICH HEINE UNIV,INST MIKROBIOL,D-40225 DUSSELDORF,GERMANY

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1993年 / 40卷 / 2-3期

关键词：

D O I：

10.1007/BF00170393

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ura3 gene of Hansenula polymorpha was cloned, sequenced and used to generate a ura3 mutant from the wild-type strain of this yeast via integrative mutagenesis. The Tn5 neomycin-resistance marker (neo) under control of the ADH1 promoter from Saccharomyces cerevisiae served as a transformation marker. The results show that gene replacement can be achieved in H. polymorpha, a yeast with a high level of non-homologous integration.

引用

页码：361 / 364

页数：4

共 26 条

[1]

AMMERER G, 1983, METHOD ENZYMOL, V101, P192

[2]

Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210

[3] EVIDENCE FOR TRANSCRIPTIONAL REGULATION OF OROTIDINE-5'-PHOSPHATE DECARBOXYLASE IN YEAST BY HYBRIDIZATION OF MESSENGER-RNA TO THE YEAST STRUCTURAL GENE CLONED IN ESCHERICHIA-COLI [J].