IDENTIFICATION OF A 2ND POLY(A) POLYMERASE IN ESCHERICHIA-COLI

被引：35

作者：

KALAPOS, MP

CAO, GJ

KUSHNER, SR

SARKAR, N

机构：

[1] BOSTON BIOMED RES INST,BOSTON,MA 02114

[2] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02115

[3] UNIV GEORGIA,DEPT GENET,ATHENS,GA 30602

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A second poly(A) polymerase (PAP II) has been identified in Escherichia coli using a strain carrying a deletion of pcnB (the structural gene for PAP I; Cao and Sarkar, 1992b) and pnp-7 (a null mutation in the structural gene for polynucleotide phosphorylase). While PAP I has a Mr of 53,000, PAP II is a smaller protein with a native Mr ˜35,000. PAP II differs from PAP I in preferring poly(A) over tRNA primers and being more thermolabile. The presence of multiple poly(A) polymerases in E. coli raises interesting questions regarding the role of polyadenylation in mRNA synthesis and decay. © 1994 Academic Press, Inc.

引用

页码：459 / 465

页数：7

共 30 条

[1] STABILIZATION OF DISCRETE MESSENGER-RNA BREAKDOWN PRODUCTS IN AMS PNP RNB MULTIPLE MUTANTS OF ESCHERICHIA-COLI K-12 [J].