RAS AMPLIFICATION IN BHK-21-CELLS PRODUCES A HOST-CELL LINE FOR FURTHER RAPID ESTABLISHMENT OF RECOMBINANT PROTEIN HYPER-PRODUCING CELL-LINES

被引：16

作者：

TERUYA, K

YANO, T

SHIRAHATA, S

WATANABE, J

OSADA, K

OHASHI, H

TACHIBANA, H

KIM, EH

MURAKAMI, H

机构：

[1] KYUSHU UNIV, GRAD SCH GENET RESOURCES TECHNOL, HIGASHI KU, FUKUOKA 812, JAPAN

[2] KIRIN BREWERY CO LTD, PHARMACEUT LAB, MAEBASHI, GUMMA 371, JAPAN

[3] KOREA UNIV, DEPT BIOTECHNOL, CHOCHIWON 339800, SOUTH KOREA

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 02期

关键词：

D O I：

10.1271/bbb.59.341

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To rapidly establish recombinant protein hyper-producing tell lines we introduced a reporter plasmid into BHK-21 cells that had been 'primed' by transfection and amplification of the eas oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.

引用

页码：341 / 344

页数：4

共 15 条

[1] P21RAS CONTRIBUTES TO HIV-1 ACTIVATION IN T-CELLS [J].