THE DIFFERENTIAL-EFFECTS OF THYROID AND GONADAL-HORMONES ON SUBSTANCE-P CONTENT IN THE ANTERIOR-PITUITARY OF THE PREPUBERTAL RAT

The effects of thyroid and gonadal status on the content of substance P in the anterior pituitary (AP-SP) were examined in prepubertal rats. A sex difference in AP-SP is evident by age 50 days [males, 287 .+-. 35 fmol/mg protein (mean .+-. SE); females, 103 .+-. 17; P < 0.05], and this difference becomes greater by 75 days (males, 543 .+-. 54; females, 146 .+-. 11.5; P < 0.01). Hypothyroidism was induced in male and female pups by giving lactating dams 0.1% methimazole (wt/vol) in their drinking water after parturition. There was a marked and significant increase in AP-SP in 21-day-old hypothyroid compared to euthyroid control pups. Male pups were made thyrotoxic by daily treatment with T4 (10 .mu.g/rat, sc) from age 8 to 15 days. AP-SP was 4 times lower in the thyrotoxic than in the euthryoid pups (P < 0.001). Rats ovariectomized at age 22 days and killed on day 35 revealed no change in AP-SP, in contrast to the rise in AP-SP in the ovariectomized adult rat. Female pups were treated with dihydrotestosterone (DHT; 50 .mu.g/day) or testosterone (50 .mu.g/day) from age 8-20 days. Neither androgen induced a change in AP-SP. Female pups which received estradiol (E2; 0.5 .mu.g/day) or testosterone (75 .mu.g/day) from age 8-20 days also had no change in AP-SP. As opposed to the lack of effect of E2 and DHT on AP-SP in female rats younger than 22 days, E2 (1 .mu.g/100 g BW daily) caused a decrease and DHT (100 .mu.g/100 g BW daily) caused an increase in AP-SP in female rats treated from 22-35 days of age [E2, 91 .+-. 6.9; DHT, 226 .+-. 31 (P < 0.05 vs. control for both); control, 154 .+-. 13]. We conclude that the responsiveness of AP-SP to alterations in thyroid status is present at the youngest age studied. In contrast, the responsiveness of AP-SP to changes in the levels of gonadal steroids is absent in the infantile period and requires a maturational process that becomes evident during the juvenile state of sexual development.