A YEAST ARTIFICIAL CHROMOSOME CONTIG ENCOMPASSING THE TYPE-1 NEUROFIBROMATOSIS GENE

被引：47

作者：

MARCHUK, DA

TAVAKKOL, R

WALLACE, MR

BROWNSTEIN, BH

TAILLONMILLER, P

FONG, CT

LEGIUS, E

ANDERSEN, LB

GLOVER, TW

COLLINS, FS

机构：

[1] UNIV MICHIGAN, DEPT HUMAN GENET, ANN ARBOR, MI 48109 USA

[2] UNIV MICHIGAN, HOWARD HUGHES MED INST, ANN ARBOR, MI 48109 USA

[3] WASHINGTON UNIV, SCH MED, CTR GENET MED, ST LOUIS, MO 63110 USA

[4] UNIV ROCHESTER, MED CTR, DEPT PEDIAT GENET, ROCHESTER, NY 14642 USA

来源：

GENOMICS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90140-N

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The yeast artificial chromosome (YAC) system (Burke et al., 1987, Science 236: 806-812) allows the direct cloning of large regions of the genome. A YAC contig map of approximately 700 kb encompassing the region surrounding the type 1 neurofibromatosis (NF1) locus on 17q11.2 has been constructed. A single YAC containing the entire NF1 locus has been constructed by homologous recombination in yeast. In the process of contig construction a novel method of YAC end rescue has been developed by YAC circularization in yeast and plasmid rescue in bacteria. YACs containing homology to the NF1 region but mapping to another chromosome have also been discovered. Sequences of portions of the homologous locus indicate that this other locus is a nonprocessed pseudogene. © 1992.

引用

页码：672 / 680

页数：9

共 36 条

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