IDENTIFICATION OF CASUARINA-FRANKIA STRAINS BY USE OF POLYMERASE CHAIN-REACTION (PCR) WITH ARBITRARY PRIMERS

被引：26

作者：

SELLSTEDT, A

WULLINGS, B

NYSTROM, U

GUSTAFSSON, P

机构：

[1] Department of Plant Physiology, University of Umeå, Umeå

[2] Department of Plant Physiology, Plant Molecular Biology Group, University of Umeå, Umeå

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 93卷 / 01期

关键词：

CASUARINACEAE; FRANKIA; IDENTIFICATION; POLYMERASE CHAIN REACTION; POLYMORPHISM;

D O I：

10.1016/0378-1097(92)90480-C

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.

引用

页码：1 / 5

页数：5

共 19 条

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