SEQUENCE AND FUNCTIONAL-CHARACTERIZATION OF FELINE INTERLEUKIN-2

被引:10
作者
COZZI, PJ
PADRID, PA
TAKEDA, J
ALEGRE, ML
YUHKI, N
LEFF, AR
机构
[1] UNIV CHICAGO,BEN MAY INST,CHICAGO,IL 60637
[2] UNIV CHICAGO,DEPT BIOCHEM & MOLEC BIOL,CHICAGO,IL 60637
[3] NCI,FREDERICK CANC RES CTR,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21701
关键词
D O I
10.1006/bbrc.1993.1926
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Since the discovery of its involvement in the pathogenesis of feline immunodeficiency virus infection ('cat AIDS') and feline leukemia virus infection, the role of feline interleukin 2 (IL-2) has been a focus of particular interest. The purpose of this study was to clone feline IL-2 cDNA, as well as synthesize bioactive recombinant feline IL-2. The isolation of cDNA encoding feline IL-2 was carried out using a PCR-based strategy and screening of a feline leukocyte cDNA library. Feline IL-2 consists of 154 amino acids including a putative signal sequence and has 81%, 69%, 60% and 64% identity to human, bovine, murine and rat IL-2, respectively. Feline IL-2 cDNA was expressed in COS-7 cells. The secreted protein has CTLL-4 murine cytotoxic T cell proliferative activity chracteristic of authentic IL-2. These data confirm the synthesis of bioactive recombinant feline IL-2. © 1993 Academic Press, Inc.
引用
收藏
页码:1038 / 1043
页数:6
相关论文
共 21 条
[1]  
BARLOUGH JE, 1991, J ACQ IMMUN DEF SYND, V4, P219
[2]   PARAMETERS OF PRODUCTION AND PARTIAL CHARACTERIZATION OF FELINE INTERLEUKIN-2 [J].
BAUER, RM ;
OLSEN, RG .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1988, 19 (3-4) :173-183
[3]   CLONING, SEQUENCE, AND EXPRESSION OF BOVINE INTERLEUKIN-2 [J].
CERRETTI, DP ;
MCKEREGHAN, K ;
LARSEN, A ;
CANTRELL, MA ;
ANDERSON, D ;
GILLIS, S ;
COSMAN, D ;
BAKER, PE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (10) :3223-3227
[4]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[5]  
COCKERELL GL, 1975, AM J VET RES, V36, P1489
[6]   MOLECULAR CHARACTERIZATION OF INTERLEUKIN-2 [J].
GILLIS, S ;
MOCHIZUKI, DY ;
CONLON, PJ ;
HEFENEIDER, SH ;
RAMTHUN, CA ;
GILLIS, AE ;
FRANK, MB ;
HENNEY, CS ;
WATSON, JD .
IMMUNOLOGICAL REVIEWS, 1982, 63 :167-209
[7]  
GOITSUKA R, 1986, JPN J VET SCI, V48, P529
[8]   A SIMPLE AND VERY EFFICIENT METHOD FOR GENERATING CDNA LIBRARIES [J].
GUBLER, U ;
HOFFMAN, BJ .
GENE, 1983, 25 (2-3) :263-269
[9]   SEQUENCE OF RAT INTERLEUKIN-2 AND ANOMALOUS BINDING OF A MOUSE INTERLEUKIN-2 CDNA PROBE TO RAT MHC CLASS-II-ASSOCIATED INVARIANT CHAIN MESSENGER-RNA [J].
MCKNIGHT, AJ ;
MASON, DW ;
BARCLAY, AN .
IMMUNOGENETICS, 1989, 30 (02) :145-147