FREE-FLOW ISOELECTRIC-FOCUSING OF HUMAN CELLULAR LYSATES AS SAMPLE PREPARATION FOR PROTEIN-ANALYSIS

被引：46

作者：

BURGGRAF, D ^{[1
]}

WEBER, G ^{[1
]}

LOTTSPEICH, F ^{[1
]}

机构：

[1] DR WEBER GMBH,ISMANING,GERMANY

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 06期

关键词：

FREE FLOW ELECTROPHORESIS; ISOELECTRIC FOCUSING; 2-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS;

D O I：

10.1002/elps.11501601169

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A whole cell lysate of human cells was separated into 80 fractions according to the pr of proteins using free flow isoelectric focusing with carrier ampholytes. The resolution of the process was highly reproducible, with an overlap of fractions of less than 30%. A protein of a faint silver stained spot in two-dimensional gel electrophoresis (2-DE) could be enriched, yielding a Coomassie blue stained spot which could be further characterized by proteinchemical methods. The enrichment of less abundant proteins from a complex crude cell extract was found to be a suitable tool for sample preparation and enrichment before applying proteins to 2-DE and reversed-phase high performance liquid chromatography.

引用

页码：1010 / 1015

页数：6

共 16 条

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