THE ADSORPTION OF LARGE PROTEINS IN ELECTROFOCUSING ON IMMOBILIZED PH GRADIENTS .1. PROTEIN SPECIFICITY AND DEPENDENCE ON IMMOBILINE AND CARRIER AMPHOLYTE CONCENTRATIONS

被引：9

作者：

FAWCETT, JS ^{[1
]}

CHRAMBACH, A ^{[1
]}

机构：

[1] NICHHD,MACROMOLEC ANAL SECT,THEORET & PHYS BIOL LAB,BLDG 10,RM 6C101,BETHESDA,MD 20892

来源：

ELECTROPHORESIS | 1988年 / 9卷 / 09期

关键词：

D O I：

10.1002/elps.1150090904

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：463 / 469

页数：7

共 14 条

[1] AVOIDING LIQUID EXUDATION ON THE SURFACE OF REHYDRATED GELS USED FOR HYBRID ISOELECTRIC-FOCUSING IN CARRIER AMPHOLYTE SUPPLEMENTED IMMOBILIZED PH GRADIENTS [J].

ALTLAND, K ;

HACKLER, R ;

ROSSMANN, U .

ELECTROPHORESIS, 1986, 7 (06) :251-259

[2] HYBRID ISOELECTRIC-FOCUSING - ADSORPTION OF PROTEINS ONTO IMMOBILIZED PH GRADIENT MATRICES AND DESORPTION BY CARRIER AMPHOLYTES [J].

ALTLAND, K ;

VONECKARDSTEIN, A ;

BANZHOFF, A ;

WAGNER, M ;

ROSSMANN, U ;

HACKLER, R ;

BECHER, P .

ELECTROPHORESIS, 1987, 8 (01) :52-62

[3] THE ADSORPTION OF LARGE PROTEINS IN ELECTROFOCUSING ON IMMOBILIZED PH GRADIENTS .2. DEPENDENCE ON THE OLIGOMERIC STATE OF IMMOBILINE [J].

FAWCETT, JS ;

VICCHIO, D ;

CHRAMBACH, A .

ELECTROPHORESIS, 1988, 9 (09) :469-474

[4] THE VOLTAGE ACROSS WIDE PH RANGE IMMOBILIZED PH GRADIENT GELS AND ITS MODULATION THROUGH THE ADDITION OF CARRIER AMPHOLYTES [J].

FAWCETT, JS ;

CHRAMBACH, A .

ELECTROPHORESIS, 1986, 7 (06) :266-272

[5] SIMPLIFIED PROCEDURE FOR THE PREPARATION OF IMMOBILIZED PH GRADIENT GELS [J].

FAWCETT, JS ;

CHRAMBACH, A .

ELECTROPHORESIS, 1986, 7 (06) :260-266

[6] A MOTOR-DRIVEN SYRINGE-TYPE GRADIENT MAKER FOR FORMING IMMOBILIZED PH GRADIENT GELS [J].

FAWCETT, JS ;

SULLIVAN, JV ;

CHIDAKEL, BE ;

CHRAMBACH, A .

ELECTROPHORESIS, 1988, 9 (05) :216-221

[7] IMMOBILIZED PH GRADIENTS IN CAPILLARY TUBES AND TWO-DIMENSIONAL GEL-ELECTROPHORESIS [J].

HOCHSTRASSER, D ;

AUGSBURGER, V ;

FUNK, M ;

APPEL, R ;

PELLEGRINI, C ;

MULLER, AF .

ELECTROPHORESIS, 1986, 7 (11) :505-511

[8]

HOCHSTRASSER D, 1986, ELECTROPHORESIS 86, P566

[9] PROTEIN PRECIPITATION INDUCED BY ALKALINE IMMOBILINES FOR ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS - CAUSES AND REMEDIES [J].

RABILLOUD, T ;

GELFI, C ;

BOSSI, ML ;

RIGHETTI, PG .

ELECTROPHORESIS, 1987, 8 (07) :305-312

[10] REMOVAL OF OLIGOMERS AND N-MERS FROM THE IMMOBILINE CHEMICALS FOR ISOELECTRIC-FOCUSING [J].

RABILLOUD, T ;

PERNELLE, JJ ;

WAHRMANN, JP ;

GELFI, C ;

RIGHETTI, PG .

JOURNAL OF CHROMATOGRAPHY, 1987, 402 :105-113

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