EFFECTS OF BORDETELLA-BRONCHISEPTICA DERMONECROTIC TOXIN ON THE STRUCTURE AND FUNCTION OF OSTEOBLASTIC CLONE MC3T3-E1 CELLS

被引：45

作者：

HORIGUCHI, Y ^{[1
]}

NAKAI, T ^{[1
]}

KUME, K ^{[1
]}

机构：

[1] KITASATO INST, VET SCI RES CTR, KASHIWA, CHIBA 277, JAPAN

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 03期

关键词：

D O I：

10.1128/IAI.59.3.1112-1116.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The effects of Bordetella bronchiseptica dermonecrotic toxin on the structure and function of a clonal osteoblastic cell line, MC3T3-E1, were investigated. The toxin induced a morphological change in the cells from a spindle shape to a spherical form with many blebs. The toxin-treated cells were viable and grew to form confluent cell layers composed of irregularly shaped cells and multinuclear cells. The toxin inhibited elevation of alkaline phosphatase activity in the cells in a dose-dependent manner at concentrations from 10 pg to 10 ng/ml. The accumulation of type I collagen in the cells was also reduced by the toxin. Since high alkaline phosphatase activity and accumulation of collagen are closely linked to differentiation of the cells into osteoblasts, it is considered likely that B. bronchiseptica dermonecrotic toxin impairs the ability of the cells to differentiate.

引用

页码：1112 / 1116

页数：5

共 26 条

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