MONOCLONAL-ANTIBODY SPECIFIC TO SIGNET RING CELLS, THE VANADOCYTES OF THE TUNICATE, ASCIDIA-SYDNEIENSIS SAMEA

被引：22

作者：

UYAMA, T ^{[1
]}

NISHIKATA, T ^{[1
]}

SATOH, N ^{[1
]}

MICHIBATA, H ^{[1
]}

机构：

[1] KYOTO UNIV,FAC SCI,DEPT ZOOL,KYOTO 606,JAPAN

来源：

JOURNAL OF EXPERIMENTAL ZOOLOGY | 1991年 / 259卷 / 02期

关键词：

D O I：

10.1002/jez.1402590208

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Ascidians are known to accumulate vanadium in their blood cells and have six to nine different types of blood cells. Among these cells, the signet ring cell has been newly identified as the vanadocyte (vanadium-containing blood cell). We have raised monoclonal antibodies specific to the signet ring cells of Ascidia sydneiensis samea for use in the identification of vanadocytes. A hybridoma cell line which secretes a monoclonal antibody specific to the signet ring cell ha been established. Immunoblotting analysis showed that the antigen recognized by the antibody is a single polypeptide of about 45 kDa. It appears that the monoclonal antibodies specific to the signet ring cells may be useful markers for studies of the process of differentiation of ascidian blood cells.

引用

页码：196 / 201

页数：6

共 30 条

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