GRAM SCALE PURIFICATION AND PREPARATION OF RABBIT LIVER ZINC METALLOTHIONEIN

被引：33

作者：

COMEAU, RD

MCDONALD, KW

TOLMAN, GL

VASAK, M

LIBERATORE, FA

机构：

[1] DUPONT CO,DEPT MED PROD 4002,331 TREBLE COVE RD,N BILLERICA,MA 01862

[2] SERAGEN INC,HOPKINTON,MA 01748

[3] MATRITECH INC,CAMBRIDGE,MA 02138

[4] CENTACOR INC,MALVERN,PA 19355

[5] UNIV ZURICH,INST BIOCHEM,CH-805 ZURICH,SWITZERLAND

来源：

PREPARATIVE BIOCHEMISTRY | 1992年 / 22卷 / 02期

关键词：

D O I：

10.1080/10826069208021365

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simplified procedure for purifying gram quantities of rabbit liver metallothionein (MT) using gel filtration and anion exchange chromatography is presented. The MT purification made use of anion exchange batch elution chromatography which greatly shortened the procedure. Quantitation techniques for use with crude and purified MT are discussed. This paper also describes the preparation of large amounts of ZnMT from Cd,ZnMT.

引用

页码：151 / 164

页数：14

共 15 条

[1] CONJUGATION OF METALLOTHIONEIN TO A MURINE MONOCLONAL-ANTIBODY [J].