DETERMINATION OF METFORMIN IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：52

作者：

HUUPPONEN, R ^{[1
]}

OJALAKARLSSON, P ^{[1
]}

ROURU, J ^{[1
]}

KOULU, M ^{[1
]}

机构：

[1] UNIV TURKU,DEPT PHARMACOL,SF-20520 TURKU 52,FINLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 583卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80564-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of metformin, an oral antidiabetic agent, in plasma is described. Plasma samples containing the internal standard, phenformin, are eluted through Amprep extraction columns before injection into the chromatographic column, packed with muBondapak phenyl. The eluent is monitored at 236 nm. At a mobile phase flow-rate of 1.35 ml/min, the retention times of metformin and phenformin are 2.8 and 5.6 min, respectively. The intra-day coefficients of variation are 1.5 and 4.3% at metformin concentrations of 0.05 and 1 mg/l, respectively.

引用

页码：270 / 273

页数：4

共 6 条

[1] ABSORPTION, DISTRIBUTION IN ORGANISM AND ELIMINATION OF METFORMIN [J].