CHICORY SEED LOT VARIETY IDENTIFICATION BY LEUCINE-AMINOPEPTIDASE AND ESTERASE ZYMOGRAM ANALYSIS

被引:7
作者
BAES, P [1 ]
VANCUTSEM, P [1 ]
机构
[1] FAC UNIV NOTRE DAME PAIX,DEPT BIOL,UNITE BIOTECHNOL,RUE BRUXELLES 61,B-5000 NAMUR,BELGIUM
关键词
D O I
10.1002/elps.11501301194
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method of identifying closely related chicory varieties has been developed using native polyacrylamide gel electrophoresis and subsequent leucine aminopeptidase and esterase staining of bulked seed sample extracts. The variety zymograms are analyzed according to a mobility-density scheme. This quick, easy and low-cost method can be a useful tool for chicory breeders.
引用
收藏
页码:885 / 886
页数:2
相关论文
共 6 条
[1]  
BAES P, 1992, PLANT BREEDING
[2]  
COOKE R J, 1986, Journal of the National Institute of Agricultural Botany, V17, P209
[4]  
Dreven F. van, 1990, Plant Varieties & Seeds, V3, P89
[5]   ANALYSIS OF BACTERIOPHAGE T7 EARLY RNAS AND PROTEINS ON SLAB GELS [J].
STUDIER, FW .
JOURNAL OF MOLECULAR BIOLOGY, 1973, 79 (02) :237-+
[6]  
Vallejos CE, 1983, ISOZYMES PLANT GENET, P469, DOI DOI 10.1016/B978-0-444-42226-2.50031-1