SUPERCOILED PLASMID SEQUENCING - ONE BUFFER FOR TEMPLATE DENATURATION, PRIMER ANNEALING, AND SYNTHESIS OF LABELED STRANDS BY REVERSE-TRANSCRIPTASE

被引：19

作者：

AGELLON, LB

CHEN, TT

机构：

[1] JOHNS HOPKINS UNIV,CHESAPEAKE BAY INST,DEPT BIOL,34TH & CHARLES STS,BALTIMORE,MD 21218

[2] MCMASTER UNIV,DEPT BIOL,HAMILTON L8S 4L8,ONTARIO,CANADA

来源：

GENE ANALYSIS TECHNIQUES | 1986年 / 3卷 / 05期

关键词：

D O I：

10.1016/0735-0651(86)90008-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：86 / 89

页数：4

共 14 条

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[8] A SYSTEM FOR SHOTGUN DNA SEQUENCING [J].

MESSING, J ;

CREA, R ;

SEEBURG, PH .

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[9] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[10]

Smith A J, 1980, Methods Enzymol, V65, P560

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