EXPRESSION OF PROTEIN-IIIA OF HUMAN ADENOVIRUS TYPE-2 IN ESCHERICHIA-COLI

被引：9

作者：

CUILLEL, M

MILLEVILLE, M

DHALLUIN, JC

机构：

[1] INSERM, U233, VIROL MOLEC LAB, 2 PL VERDUN, F-59045 LILLE, FRANCE

[2] INST MAX VON LAUE PAUL LANGEVIN, EUROPEAN MOLEC BIOL LAB, F-38042 GRENOBLE, FRANCE

来源：

GENE | 1987年 / 55卷 / 2-3期

关键词：

D O I：

10.1016/0378-1119(87)90289-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a plasmid encoding the protein IIIa gene of human adenovirus type 2. The gene was expressed under the control of the hybrid tac (trp-lac) promoter; the protein was synthesized at levels up to 5% of newly synthesized protein after IPTG induction. The protein IIIa produced in Escherichia coli has an apparent Mr on sodium dodecyl sulfate-polyacrylamide gels of 67 kDa, and was revealed with anti-adenovirus serum in Western blotting. The protein IIIa produced in bacteria was phosphorylated in the presence of [.gamma.-32P]ATP.

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页码：295 / 301

页数：7

相关论文

共 20 条

[1] PROTEIN-KINASE ASSOCIATED WITH ADENOVIRUS TYPE-2 [J].

AKUSJARVI, G ;

PHILIPSON, L ;

PETTERSSON, U .

VIROLOGY, 1978, 87 (02) :276-286

[2] VECTORS BEARING A HYBRID TRP-LAC PROMOTER USEFUL FOR REGULATED EXPRESSION OF CLONED GENES IN ESCHERICHIA-COLI [J].

AMANN, E ;

BROSIUS, J ;

PTASHNE, M .

GENE, 1983, 25 (2-3) :167-178

[3] PROCESSING OF ADENOVIRUS 2-INDUCED PROTEINS [J].

ANDERSON, CW ;

BAUM, PR ;

GESTELAND, RF .

JOURNAL OF VIROLOGY, 1973, 12 (02) :241-252

[4]

[Anonymous], 1972, Experiments in molecular genetics, DOI papers2://publication/uuid/28F839D6-A272-4D50-9114-D2769DE72B38

[5]

BLAIR GE, 1978, VIROLOGY, V86, P157, DOI 10.1016/0042-6822(78)90016-8

[6] HUMAN ADENOVIRUS TYPE-2 PROTEIN-IIIA .2. MATURATION AND ENCAPSIDATION [J].

BOUDIN, ML ;

DHALLUIN, JC ;

COUSIN, C ;

BOULANGER, P .

VIROLOGY, 1980, 101 (01) :144-156

[7]

DEBOER HA, 1983, P NATL ACAD SCI-BIOL, V80, P21

[8] MOLECULAR-WEIGHT OF ADENOVIRUS SEROTYPE-2 CAPSOMERS - A NEW CHARACTERIZATION [J].

DEVAUX, C ;

ZULAUF, M ;

BOULANGER, P ;

JACROT, B .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 156 (04) :927-939

[9] TEMPERATURE-SENSITIVE MUTANT OF ADENOVIRUS TYPE-2 BLOCKED IN VIRION ASSEMBLY - ACCUMULATION OF LIGHT INTERMEDIATE PARTICLES [J].

DHALLUIN, JC ;

MILLEVILLE, M ;

BOULANGER, PA ;

MARTIN, GR .

JOURNAL OF VIROLOGY, 1978, 26 (02) :344-356

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+