GLYCOL METHACRYLATE EMBEDDING OF ALGINATE-POLYLYSINE MICROENCAPSULATED PANCREATIC-ISLETS

被引：14

作者：

FRITSCHY, WM ^{[1
]}

GERRITS, PO ^{[1
]}

WOLTERS, GHJ ^{[1
]}

PASMA, A ^{[1
]}

VANSCHILFGAARDE, R ^{[1
]}

机构：

[1] UNIV GRONINGEN,DEPT ANAT & EMBRYOL,9713 EZ GRONINGEN,NETHERLANDS

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1995年 / 70卷 / 04期

关键词：

GLYCOL METHACRYLATE; GMA; RESIN; ALGINATE; POLYLYSINE; MICROENCAPSULATION; PANCREATIC ISLETS;

D O I：

10.3109/10520299509107311

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method for processing and embedding alginate-polylysine microencapsulated pancreatic tissue in glycol methacrylate resin (GMA) is described. Fixation in 4% phosphate buffered formaldehyde, processing in ascending concentrations of glycol methacrylate monomer and embedding in Technovit 7100 results in well preserved morphological details of hydrogels, hydrogel-cell interfaces, and encapsulated pancreatic tissue, Routine staining with Loeffler's methylene blue, hematoxylin and eosin, and Romanovsky-Giemsa gave excellent images of the GMA embedded alginate polylysine membrane and tissues allowing cells on the outside of the capsule to be analyzed effectively as part of the foreign body reaction against the capsule membrane.

引用

页码：188 / 193

页数：6

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