MOLECULAR-CLONING AND CHARACTERIZATION OF THE GENES FOR A NON-FIMBRIAL ADHESIN FROM ESCHERICHIA-COLI

被引:13
作者
HALES, BA
BEVERLEYCLARKE, H
HIGH, NJ
JANN, K
PERRY, R
GOLDHAR, J
BOULNOIS, GJ
机构
[1] UNIV LEICESTER, DEPT MICROBIOL, MED SCI BLDG, LEICESTER LE1 7RH, ENGLAND
[2] MAX PLANCK INST IMMUNBIOL, D-7800 FREIBURG, FED REP GER
[3] SACKLER SCH MED, DEPT HUMAN MICROBIOL, TEL AVIV, ISRAEL
基金
英国惠康基金;
关键词
D O I
10.1016/0882-4010(88)90076-9
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A non-fimbrial adhesin (NFA-1) from the uropathogenic Escherichia coli strain 827 responsible for agglutination of human erythrocytes was cloned using the cos 4 cosmid vector. A clone was isolated which promoted haemagglutination and showed the same biological properties as the adhesin produced by the wild type strain. Both express adhesin at 37.degree. C, but not 18.degree. C nor in the presence of 1% glucose. Adhesin purified from the clone formed high molecular weight aggregates which were resolved to the 21K dalton subunit protein seen in the wild type strain on denaturation. Binding to human kidney cells by the clone and the wild type E. coli, from which the genes were cloned, were compared in an ELISA assay and shown to be the same. The genes for the adhesin were isolated on a 15.5 kilobase BamHI-EcoRI fragment which was subjected to .gamma..delta. mutagenesis. The NFA-1 operon was localized to a 6.5 kb region of this fragment.
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页码:9 / 17
页数:9
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