INVIVO STUDY ON MEDULLARY H+-SENSITIVE NEURONS

被引:26
作者
KOGO, N
ARITA, H
机构
[1] Basic Medical Sciences Inst., University of Tsukuba, Tsukuba
关键词
cat; central chemoreceptors; control of breathing; micro pressure ejection; nucleus tractus solitarii; ventrolateral medulla;
D O I
10.1152/jappl.1990.69.4.1408
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Using the micro pressure ejection technique, we examined responses of medullary neurons with nonphasic discharges (164 units) to direct application of acidified mock cerebrospinal fluid (CSF, pH 6.85-7.05) in decerebrated spontaneously breathing cats. We found 16 H+-sensitive cells; they were excited promptly on application of ~500 pl of acidified mock CSF in the vicinity of the neuron under investigation, whereas they were unaffected by microejection of the control mock CSF (pH 7.25-7.60). Of the 16 H+-sensitive cells, 10 units were further found to be excited by transcapillary stimulation of the central chemoreceptors by using a method of intravertebral arterial injection of CO2-saturated saline. The discharges increased in a similar time course to that of ventilatory augmentation. Distributions of these 10 specific H+ -sensitive cells were found in the vicinity of nucleus tractus solitarii as well as deep in the ventrolateral medulla. The present results suggest a possibility that pH-dependent central chemoreceptors, if any, would be located in two distinct medullary regions described in this study.
引用
收藏
页码:1408 / 1412
页数:5
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