HIGH-AFFINITY BINDING OF FIBRONECTIN TO CULTURED KUPFFER CELLS

被引:17
作者
CARDARELLI, PM
BLUMENSTOCK, FA
MCKEOWNLONGO, PJ
SABA, TM
MAZURKIEWICZ, JE
DIAS, JA
机构
[1] UNION UNIV,DEPT PHYSIOL,BOX A-134,47 NEW SCOTLAND AVE,ALBANY,NY 12208
[2] UNION UNIV,DEPT CELL BIOL,ALBANY,NY 12208
[3] UNION UNIV,DEPT BIOCHEM,ALBANY,NY 12208
[4] UNION UNIV,DEPT ANAT,ALBANY,NY 12208
[5] WADSWORTH CTR LABS & RES,DEPT BIOMED SCI,ALBANY,NY
关键词
erythrocytes; gelatin; liver;
D O I
10.1002/jlb.48.5.426
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Hepatic Kupffer cells are a major component of the reticuloendothelial or macrophage system. They were the first phagocytic cell type whose phagocytosis was shown to be influenced by plasma fibronectin, a dimeric opsonic glycoprotein. In the current study, the binding of soluble radioiodinated fibronectin purified from rat serum to isolated rat hepatic Kupffer cells was investigated using a cultured Kupffer cell monolayer technique. Binding was specific, since unlabeled purified fibronectin competed in a dose-dependent manner with the 125I-fibronectin for binding to the Kupffer cells. Addition of gelatin enhanced the binding of 125I-fibronectin to Kupffer cells. The phagocytosis of gelatinized-coated red cells by Kupffer cells was increased either by preopsonizing the target particles with purified fibronectin or by the addition of purified fibronectin to the culture medium. In contrast, exposure of the Kupffer cells to medium containing purified fibronectin followed by wash-removal of the fibronectin did not increase the uptake of gelatin-coated red blood cells, even though fibronectin was detected on the surface of the Kupffer cells by immunofluorescence. Trypsinized monolayers expressed decreased capacity to bind 125I-fibronectin as well as fibronectin-coated sheep erythrocytes. The binding of 125I-fibronectin-gelatin complexes was inhibited by excess unlabeled fibronectin. We calculated that specific high-affinity (K(d) = 7.46 x 10-9 M) binding sites for fibronectin exist on Kupffer cells. There are approximately 2,800-3,500 binding sites or putative 'fibronectin receptors' per Kupffer cell. These sites appear to mediate the enhanced phagocytosis of gelatin-coated particles opsonized by fibronectin.
引用
收藏
页码:426 / 437
页数:12
相关论文
共 32 条
  • [1] MACROPHAGE PHAGOCYTOSIS - ANALYSIS OF PARTICLE BINDING AND INTERNALIZATION
    BESTERMAN, JM
    AIRHART, JA
    LOW, RB
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (05): : C339 - C346
  • [2] RECEPTORS FOR COLD-INSOLUBLE GLOBULIN (PLASMA FIBRONECTIN) ON HUMAN-MONOCYTES
    BEVILACQUA, MP
    AMRANI, D
    MOSESSON, MW
    BIANCO, C
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1981, 153 (01) : 42 - 60
  • [3] Blumenstock F A, 1979, Adv Shock Res, V2, P55
  • [4] BLUMENSTOCK FA, 1981, J RETICULOENDOTH SOC, V30, P61
  • [5] FIBRONECTIN RECEPTORS OF PHAGOCYTES - CHARACTERIZATION OF THE ARG-GLY-ASP BINDING-PROTEINS OF HUMAN-MONOCYTES AND POLYMORPHONUCLEAR LEUKOCYTES
    BROWN, EJ
    GOODWIN, JL
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (03) : 777 - 793
  • [6] FIBRONECTIN-ENHANCED ATTACHMENT OF GELATIN-COATED ERYTHROCYTES TO ISOLATED HEPATIC KUPFFER CELLS
    CARDARELLI, PM
    BLUMENSTOCK, FA
    SABA, TM
    ROURKE, FJ
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 1984, 36 (04) : 477 - 492
  • [7] MECHANISM OF ACUTE DEPLETION OF PLASMA FIBRONECTIN FOLLOWING THERMAL-INJURY IN RATS - APPEARANCE OF A GELATIN-LIKE LIGAND IN PLASMA
    DENO, DC
    MCCAFFERTY, MH
    SABA, TM
    BLUMENSTOCK, FA
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1984, 73 (01) : 20 - 34
  • [8] KINETICS OF ENDOGENOUSLY LABELED PLASMA FIBRONECTIN - INCORPORATION INTO TISSUES
    DENO, DC
    SABA, TM
    LEWIS, EP
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1983, 245 (04): : R564 - R575
  • [9] PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY
    GREENWOOD, FC
    HUNTER, WM
    [J]. BIOCHEMICAL JOURNAL, 1963, 89 (01) : 114 - &
  • [10] FIBRONECTIN-MEDIATED UPTAKE OF GELATIN-COATED LATEX-PARTICLES BY PERITONEAL-MACROPHAGES
    GUDEWICZ, PW
    MOLNAR, J
    LAI, MZ
    BEEZHOLD, DW
    SIEFRING, GE
    CREDO, RB
    LORAND, L
    [J]. JOURNAL OF CELL BIOLOGY, 1980, 87 (02) : 427 - 433