THE PRIMARY STRUCTURE OF 6 LEUCINE ISOACCEPTOR TRANSFER-RNAS OF YELLOW LUPIN SEEDS - THE STRUCTURAL REQUIREMENTS FOR AMBER TRANSFER-RNA SUPPRESSOR ACTIVITY

Six tRNALeu isoacceptors from yellow lupin seeds were purified, sequenced, and their readthrough properties over the UAG stop codon were tested using TMV RNA as a messenger. The tested tRNAsLeu did not show amber suppressor activity. The partial structure of tRNAGln, a minor species in yellow lupin, was also determined. Comparison of the nucleotide sequence of all known isoacceptors of tRNATyr, tRNAGln and tRNALeu from plants, mammals and ciliates enabled us to find general structural requirements for tRNA to be a UAG suppressor. From the partial sequence of lupin tRNAGln we suggest that it will have readthrough properties. © 1990.