OVEREXPRESSION OF EXTRACELLULAR SUCRASE (SACC) OF ZYMOMONAS-MOBILIS IN ESCHERICHIA-COLI

被引：3

作者：

KANNAN, R

PITCHAIMANI, K

GUNASEKARAN, P

AITABDELKADER, N

BARATTI, J

机构：

[1] FAC SCI LUMINY, BIOTECHNOL LAB, F-13299 MARSEILLE 9, FRANCE

[2] MADURAI KAMARAJ UNIV, SCH BIOL SCI, DEPT MICROBIAL TECHNOL, MADURAI 625021, TAMIL NADU, INDIA

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 133卷 / 1-2期

关键词：

ZYMOMONAS MOBILIS; SUCRASE; OVEREXPRESSION; ESCHERICHIA COLI;

D O I：

10.1016/0378-1097(95)00327-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The extracellular sucrase (SacC) gene of Zymomonas mobilis was overexpressed in Escherichia coli BL21 using the T7 polymerase expression system. A low cell density induction method was designed to have maximum expression, and the conditions (IPTG concentration, ampicillin addition) were optimised to overexpress to the level of more than 60% of the total cellular protein representing SacC protein.

引用

页码：29 / 33

页数：5

共 17 条

[1]

AITABDELKADER N, 1993, THESIS U AIX MARSEIL

[2] ZYMOMONAS-MOBILIS - A BACTERIUM FOR ETHANOL-PRODUCTION [J].