DEVELOPMENT OF BIOMECHANICAL PROPERTIES AND MORPHOGENESIS OF IN-VITRO TISSUE ENGINEERED CARTILAGE

被引：104

作者：

MA, PX

SCHLOO, B

MOONEY, D

LANGER, R

机构：

[1] MIT,DEPT CHEM ENGN,CAMBRIDGE,MA 02139

[2] DEBORAH HEART & LUNG CTR,DEPT PATHOL,BROWNS MILLS,NJ 08015

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 1995年 / 29卷 / 12期

关键词：

D O I：

10.1002/jbm.820291215

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Neocartilage was engineered by culturing bovine chondrocytes on poly(glycolic acid) (PGA) fibrous nonwoven scaffolds. The biomechanical properties and morphologies of the PGA-chondrocyte constructs were studied over 12 weeks of in vitro culture. PGA scaffolds without cells lost their mechanical strength and structural integrity between week 2 and week 3 in culture. The thickness of the PGA-chondrocyte constructs decreased by 35% during the first 3 weeks, but the thickness increased from week 3 to week 9 to a thickness 42% higher than that of the starting scaffolds, which was then maintained. Safranin O staining of PGA-chondrocyte constructs revealed increasing proteoglycan formation over time. The compressive modules of PGA-chondrocyte constructs increased with in vitro culture time, and reached the same order of magnitude as that of normal bovine cartilage at week 9. The aggregate modulus of the PGA-chondrocyte constructs decreased by 57% over the first 2 weeks but then increased, reaching the same order of magnitude as normal bovine cartilage at week 12. The apparent permeability of the PGA-chondrocyte constructs, which was initially four orders of magnitude above that of normal cartilage, decreased between weeks 1 and 3 and thereafter remained the same order of magnitude as that measured for normal cartilage. (C) 1995 John Wiley & Sons, Inc.

引用

页码：1587 / 1595

页数：9

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