MAPPING OF A DOMINANT IMMUNOGENIC REGION OF SYNAPTOPHYSIN, A MAJOR MEMBRANE-PROTEIN OF SYNAPTIC VESICLES

被引：29

作者：

KNAUS, P ^{[1
]}

BETZ, H ^{[1
]}

机构：

[1] UNIV HEIDELBERG,ZENTRUM MOLEK BIOL,NEUENHEIMER FELD 282,W-6900 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1990年 / 261卷 / 02期

关键词：

(Rat brain); Antigenic epitope; Site-directed mutagenesis; Synaptic vesicle; Synaptophysin;

D O I：

10.1016/0014-5793(90)80591-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synaptophysin is a major integral membrane protein of synaptic vesicles. Its transmembrane topology deduced from the cDNA sequence predicts 4 transmembrane regions and a carboxy-terminal cytoplasmic tail containing a characteristic pentapeptide repeat structure. The monoclonal antibody (mAb), SY38, binds to a cytoplasmic domain of synaptophysin. By using fusion proteins corresponding to truncated forms of the cytoplasmic tail, its epitope was located to a flexible segment in the center of the repeat structure. Four other mAbs (c7.1, c7.2, c7.3, c7.4) share the same epitope, which thus emerges as the major immunogenic region of this membrane protein. © 1990.

引用

页码：358 / 360

页数：3

共 13 条

[1]

COWAN D, 1989, IN PRESS MOL BRAIN R

[2] A 38,000-DALTON MEMBRANE-PROTEIN (P38) PRESENT IN SYNAPTIC VESICLES [J].

JAHN, R ;

SCHIEBLER, W ;

OUIMET, C ;

GREENGARD, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (12) :4137-4141

[3]

JOHNSTON PA, 1989, J BIOL CHEM, V264, P1268

[4] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[5] SYNAPTOPHYSIN - MOLECULAR-ORGANIZATION AND MESSENGER-RNA EXPRESSION AS DETERMINED FROM CLONED CDNA [J].

LEUBE, RE ;

KAISER, P ;

SEITER, A ;

ZIMBELMANN, R ;

FRANKE, WW ;

REHM, H ;

KNAUS, P ;

PRIOR, P ;

BETZ, H ;

REINKE, H ;

BEYREUTHER, K ;

WIEDENMANN, B .

EMBO JOURNAL, 1987, 6 (11) :3261-3268

[6]

REHM HH, 1986, EMBO J, V2, P535

[7] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[8] 5'-3' EXONUCLEASES IN PHOSPHOROTHIOATE-BASED OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS [J].

SAYERS, JR ;

SCHMIDT, W ;

ECKSTEIN, F .

NUCLEIC ACIDS RESEARCH, 1988, 16 (03) :791-802

[9] CHARACTERIZATION OF FOOT-AND-MOUTH-DISEASE VIRUS GENE-PRODUCTS WITH ANTISERA AGAINST BACTERIALLY SYNTHESIZED FUSION PROTEINS [J].

STREBEL, K ;

BECK, E ;

STROHMAIER, K ;

SCHALLER, H .

JOURNAL OF VIROLOGY, 1986, 57 (03) :983-991

[10] A SYNAPTIC VESICLE PROTEIN WITH A NOVEL CYTOPLASMIC DOMAIN AND 4 TRANSMEMBRANE REGIONS [J].

SUDHOF, TC ;

LOTTSPEICH, F ;

GREENGARD, P ;

MEHL, E ;

JAHN, R .

SCIENCE, 1987, 238 (4830) :1142-1144

← 1 2 →