POLYVINYLPYRROLIDONE-AGAROSE GEL-ELECTROPHORESIS PURIFICATION OF POLYMERASE CHAIN REACTION-AMPLIFIABLE DNA FROM SOILS

被引：130

作者：

YOUNG, CC ^{[1
]}

BURGHOFF, RL ^{[1
]}

KEIM, LG ^{[1
]}

MINAKBERNERO, V ^{[1
]}

LUTE, JR ^{[1
]}

HINTON, SM ^{[1
]}

机构：

[1] EXXON RES & ENGN CO, ROUTE 22 E, ANNANDALE, NJ 08801 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 06期

关键词：

D O I：

10.1128/AEM.59.6.1972-1974.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards the electrophoretic mobility of denaturing phenolic compounds so that they do not comigrate with nucleic acids.

引用

页码：1972 / 1974

页数：3

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