LANGERHANS CELLS ARE THE MAJOR SOURCE OF MESSENGER-RNA FOR IL-1-BETA AND MIP-1-ALPHA AMONG UNSTIMULATED MOUSE EPIDERMAL-CELLS

被引:89
作者
MATSUE, H [1 ]
CRUZ, PD [1 ]
BERGSTRESSER, PR [1 ]
TAKASHIMA, A [1 ]
机构
[1] UNIV TEXAS, SW MED CTR, DEPT DERMATOL, 5323 HARRY HINES BLVD, DALLAS, TX 75235 USA
关键词
D O I
10.1111/1523-1747.ep12667296
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Immunocompetent cells of the epidermis can interact by the elaboration and recognition of cytokines. Although much new information has been reported concerning the cytokines secreted by keratinocytes, little is known about cytokines derived from Langerhans cells (LC). To address this deficiency, we examined cytokine mRNA profiles in different epidermal preparations from BALB/c mice, taking advantage of the sensitive technique of polymerase chain reaction (PCR), after reverse transcription of mRNA. In assays of epidermal sheets separated from dermis by ammonium thiocyanate, mRNA for IL-1alpha, IL-1beta, IL-6, IL-7, tumor necrosis factor alpha (TNFalpha), TNFbeta, granulocyte macrophage/colony-stimulating factor (GM-CSF), and macrophage inflammatory protein-1alpha (MIP-1alpha) were unequivocally present. By contrast, faint bands were detected for IL-4, IL-5, and interferon gamma (IFNgamma), and no PCR signal was detected for IL-2. Importantly, assays of epidermal cells (EC) dissociated with trypsin revealed similar mRNA profiles. To determine the effects of cell isolation, fluorescence-activated cell sorter (FACS)-purified Ia- EC were first analyzed; all of the previously cited cytokine mRNA were present except for IL-1beta and MIP-1alpha. EC depleted of LC by a second technique, lysis using anti-Ia monoclonal antibody and complement, revealed similar profiles, with substantially reduced PCR signals for IL-1beta and MIP-1alpha. Finally, FACS-purified LC (Ia+ EC) clearly expressed IL-1beta and MIP-1alpha mRNA, a finding that was verified by Southern blotting using internal oligo probes. We conclude that these cell-isolation procedures did not produce substantial alterations in basal mRNA profiles and that LC are the principal source of mRNA for IL-1beta and MIP-1alpha among unstimulated EC in mice.
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页码:537 / 541
页数:5
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