RENIN RELEASE FROM PERMEABILIZED JUXTAGLOMERULAR CELLS IS STIMULATED BY CHLORIDE BUT NOT BY LOW-CALCIUM

被引:26
作者
JENSEN, BL [1 ]
SKOTT, O [1 ]
机构
[1] UNIV COPENHAGEN,PANUM INST,INST MED PHYSIOL,DIV PATHOPHYSIOL,DK-2200 COPENHAGEN,DENMARK
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1994年 / 266卷 / 04期
关键词
ANIONS; IODIDE; NITRATE; DIGITONIN; EXOCYTOSIS;
D O I
10.1152/ajprenal.1994.266.4.F604
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The intracellular concentrations of calcium and chloride have been suggested to be involved in the control of renin secretion from juxtaglomerular (JG) cells. We have tested these propositions on permeabilized JG cells. Rat glomeruli with attached JG cells were isolated by the magnetic iron technique, superfused, and permeabilized by 20 mu M digitonin for 12 min. The calcium concentration was varied with Ca ethylene glycol-bis (beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) buffers [0 (5 mM EGTA without calcium), 17, 73, 170, 440, or 700 nM and 1.5, 15 or 150 mu M]. These maneuvers had no effect on renin release, while 1.5 mM calcium caused a stimulation, which was not inhibited by 50 mM sucrose. Isosmotic increases in the chloride concentration to 25, 60, and 132 mM resulted in prompt stimulations of renin release. Similarly, iodide and nitrate stimulated renin release. We conclude that renin release from permeabilized JG cells is unaffected by calcium concentrations in the nano- and micromolar range, while the release is stimulated by chloride or other permeant anions. We suggest that in intact JG cells an increase in calcium inhibits renin release through activation of chloride channels followed by a drop in the intracellular chloride concentration. The stimulation caused by the high calcium concentration may be a toxic effect or may be due to stimulation of the fusion between granules and cell membrane in a way analogous to other secretory cells.
引用
收藏
页码:F604 / F611
页数:8
相关论文
共 34 条
[1]   CALCIUM REQUIREMENTS FOR SECRETION IN BOVINE CHROMAFFIN CELLS [J].
AUGUSTINE, GJ ;
NEHER, E .
JOURNAL OF PHYSIOLOGY-LONDON, 1992, 450 :247-271
[2]   CHARACTERISTICS OF RENIN RELEASE FROM ISOLATED SUPERFUSED GLOMERULI INVITRO [J].
BLENDSTRUP, K ;
LEYSSAC, PP ;
POULSEN, K ;
SKINNER, SL .
JOURNAL OF PHYSIOLOGY-LONDON, 1975, 246 (03) :653-672
[3]   IDENTIFICATION AND REGULATION OF RENIN IN HUMAN CULTURED MESANGIAL CELLS [J].
CHANSEL, D ;
DUSSAULE, JC ;
ARDAILLOU, N ;
ARDAILLOU, R .
AMERICAN JOURNAL OF PHYSIOLOGY, 1987, 252 (01) :F32-F38
[4]   2ND MESSENGERS IN RENIN SECRETION [J].
CHURCHILL, PC .
AMERICAN JOURNAL OF PHYSIOLOGY, 1985, 249 (02) :F175-F184
[5]  
GOMPERTS BD, 1990, ANNU REV PHYSIOL, V52, P591
[6]   ACTIVATION OF PERMEABILIZED NEUTROPHILS - ROLE OF ANIONS [J].
GRINSTEIN, S ;
FURUYA, W ;
DOWNEY, GP .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (01) :C78-C85
[7]  
HIGASHIJIMA T, 1987, J BIOL CHEM, V262, P3597
[8]   OSMOTICALLY SENSITIVE RENIN RELEASE FROM PERMEABILIZED JUXTAGLOMERULAR CELLS [J].
JENSEN, BL ;
SKOTT, O .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (01) :F87-F95
[9]  
KUROKAWA K, 1991, CONTRIB NEPHROL, V95, P76
[10]   LACK OF DIRECT EVIDENCE FOR A FUNCTIONAL-ROLE OF VOLTAGE-OPERATED CALCIUM CHANNELS IN JUXTAGLOMERULAR CELLS [J].
KURTZ, A ;
SKOTT, O ;
CHEGINI, S ;
PENNER, R .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1990, 416 (03) :281-287