CAMP-INDUCED MORPHOLOGICAL-CHANGES IN AN IMMORTALIZED SCHWANN-CELL LINE - A PRELUDE TO DIFFERENTIATION

被引:11
作者
DEDEYNE, PG
DEVRIES, GH
BIGBEE, JW
机构
[1] VIRGINIA COMMONWEALTH UNIV, DEPT ANAT, RICHMOND, VA 23298 USA
[2] VIRGINIA COMMONWEALTH UNIV, DEPT BIOCHEM & MOLEC BIOPHYS, RICHMOND, VA 23298 USA
来源
CELL MOTILITY AND THE CYTOSKELETON | 1994年 / 29卷 / 01期
关键词
PROLIFERATION; LARGE T ANTIGEN; PERIPHERAL NERVOUS SYSTEM; CYTOSKELETON; MICROTUBULES; MYELINATION;
D O I
10.1002/cm.970290103
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Schwann cells (SC), the myelinating cells of the peripheral nervous system, show a remarkable capacity to switch from a differentiated state to a proliferative state both during development and peripheral nerve regeneration. In order to better understand the regulatory mechanisms involved with this change we are studying a Schwann cell line transfected with the SV-40 large T gene (TSC). Serum-free medium combined with elevating intra-cellular cAMP levels produced a slower proliferating TSC whose morphology changed from pleiomorphic to process bearing, reminiscent of primary SC in culture. This change was abrogated by colcemid but was unaltered by cytochalasin D, indicating a major role for microtubules. Ultrastructural studies demonstrated numerous microtubules in the cellular extensions which correlated with strong immunocytochemical staining for tubulin in the processes. Analysis of cytoskeletal fractions from the treated cells revealed a greater proportion of tubulin in the polymerized state compared with untreated cells which closely resembled the distribution in primary SC. The cytoskeletal changes observed in the TSC as a result of elevating the intra-cellular cAMP levels may reflect the earliest cellular changes in the induction of myelination. (C) 1994 Wiley-Liss, Inc.
引用
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页码:20 / 28
页数:9
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