CELL LINE-SPECIFIC DIFFERENCES IN THE CONTROL OF CELL-CYCLE PROGRESSION IN THE ABSENCE OF MITOSIS

被引:240
作者
KUNG, AL [1 ]
SHERWOOD, SW [1 ]
SCHIMKE, RT [1 ]
机构
[1] STANFORD UNIV,DEPT BIOL SCI,STANFORD,CA 94305
关键词
Colcemid; cyclin B; nocodazole; p34(cdc2); taxol;
D O I
10.1073/pnas.87.24.9553
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
This paper reports that there are major differences between mammalian cell lines in the propensity to progress into subsequent cell cycles when mitosis is inhibited with agents that disrupt the assembly of the mitotic spindle apparatus (Colcemid, nocodazole, and taxol). Human HeLa S3 cells, which represent one extreme, remain arrested in mitosis, with elevated levels of cyclin B and p34(cdc2) kinase activity. In Chinese hamster ovary cells, at the other extreme, the periodic rise and fall of cyclin B levels and p34(cdc2) kinase activity is only transiently inhibited in the absence of mitosis. The cells progress into subsequent cell cycles, without dividing, resulting in serial doublings of cellular DNA content. In general, the propensity to progress into subsequent cell cycles in the absence of mitosis appears to be species related, such that human cell lines remain permanently blocked in a mitotic state, whereas rodent cell lines are only transiently inhibited when spindle assembly is disrupted. We interpret these results to indicate that in mammalian cell lines there exists a checkpoint which serves to couple cell cycle progression to the completion of certain karyokinetic events. Furthermore, either such a checkpoint exists in some cell lines but not in others or the stringency of the control mechanism varies among different cell lines.
引用
收藏
页码:9553 / 9557
页数:5
相关论文
共 11 条
[1]   MUTATIONS IN ALPHA-TUBULIN AND BETA-TUBULIN AFFECT SPINDLE FORMATION IN CHINESE-HAMSTER OVARY CELLS [J].
ABRAHAM, I ;
MARCUS, M ;
CABRAL, F ;
GOTTESMAN, MM .
JOURNAL OF CELL BIOLOGY, 1983, 97 (04) :1055-1061
[2]   P34CDC2 IS LOCATED IN BOTH NUCLEUS AND CYTOPLASM - PART IS CENTROSOMALLY ASSOCIATED AT G2/M AND ENTERS VESICLES AT ANAPHASE [J].
BAILLY, E ;
DOREE, M ;
NURSE, P ;
BORNENS, M .
EMBO JOURNAL, 1989, 8 (13) :3985-3995
[3]   MUTATION OF FISSION YEAST-CELL CYCLE CONTROL GENES ABOLISHES DEPENDENCE OF MITOSIS ON DNA-REPLICATION [J].
ENOCH, T ;
NURSE, P .
CELL, 1990, 60 (04) :665-673
[4]   CHECKPOINTS - CONTROLS THAT ENSURE THE ORDER OF CELL-CYCLE EVENTS [J].
HARTWELL, LH ;
WEINERT, TA .
SCIENCE, 1989, 246 (4930) :629-634
[5]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[6]   DRIVING THE CELL-CYCLE - M-PHASE KINASE, ITS PARTNERS, AND SUBSTRATES [J].
LEWIN, B .
CELL, 1990, 61 (05) :743-752
[7]   S PHASE-SPECIFIC SYNTHESIS OF DIHYDROFOLATE-REDUCTASE IN CHINESE-HAMSTER OVARY CELLS [J].
MARIANI, BD ;
SLATE, DL ;
SCHIMKE, RT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (08) :4985-4989
[8]   DOMINOES AND CLOCKS - THE UNION OF 2 VIEWS OF THE CELL-CYCLE [J].
MURRAY, AW ;
KIRSCHNER, MW .
SCIENCE, 1989, 246 (4930) :614-621
[9]   UNIVERSAL CONTROL MECHANISM REGULATING ONSET OF M-PHASE [J].
NURSE, P .
NATURE, 1990, 344 (6266) :503-508
[10]   ISOLATION OF A HUMAN CYCLIN CDNA - EVIDENCE FOR CYCLIN MESSENGER-RNA AND PROTEIN-REGULATION IN THE CELL-CYCLE AND FOR INTERACTION WITH P34-CDC2 [J].
PINES, J ;
HUNTER, T .
CELL, 1989, 58 (05) :833-846