ANALYSIS OF A HISTONE H2A VARIANT FROM FISSION YEAST - EVIDENCE FOR A ROLE IN CHROMOSOME STABILITY

被引：77

作者：

CARR, AM

DORRINGTON, SM

HINDLEY, J

PHEAR, GA

AVES, SJ

NURSE, P

机构：

[1] IMPERIAL CANC RES FUND, LONDON WC2 A3PX, ENGLAND

[2] UNIV BRISTOL, DEPT BIOCHEM, BRISTOL BS8 IRD, AVON, ENGLAND

[3] UNIV EXETER, DEPT BIOL SCI, EXETER EX4 4QG, DEVON, ENGLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1994年 / 245卷 / 05期

关键词：

SCHIZOSACCHAROMYCES POMBE; HISTONE H2A VARIANT; CHROMOSOME STABILITY; H2A.F/Z;

D O I：

10.1007/BF00282226

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and characterised the pht1 gene from the fission yeast Schizosaccharomyces pombe. The sequence of the predicted translation product has revealed a striking similarity to the family of H2A.F/Z histone variant proteins, which have been found in a variety of different organisms. Cells deleted for the pht1 gene locus grow slowly, exhibit an altered colony morphology, increased resistance to heat shock and show a significant decrease in the fidelity of segregation of an S. pombe minichromosome. We propose that the histone H2A variant encoded by the pht1 gene is important for chromosomal structure and function, possibly including a role in controlling the fidelity of chromosomal segregation during mitosis.

引用

页码：628 / 635

页数：8

共 44 条

[1] HISTONE VARIANTS SPECIFIC TO THE TRANSCRIPTIONALLY ACTIVE, AMITOTICALLY DIVIDING MACRONUCLEUS OF THE UNICELLULAR EUCARYOTE, TETRAHYMENA-THERMOPHILA [J].