EFFECTS OF CULTURE CONDITIONS ON ISOLATED MICROSPORE RESPONSE OF BARLEY CULTIVAR IGRI

被引：27

作者：

CISTUE, L ^{[1
]}

ZIAUDDIN, A ^{[1
]}

SIMION, E ^{[1
]}

KASHA, KJ ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT CROP SCI,GUELPH,ON N1G 2W1,CANADA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1995年 / 42卷 / 02期

关键词：

HORDEUM VULGARE; FICOLL; GELRITE; MICROSPORE CULTURE;

D O I：

10.1007/BF00034234

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pretreatment of anthers in mannitol prior to isolation of microspores by glass rod homogenization was effective for in vitro induction of embryogenesis in barley cv. Igri. A procedure for separation of viable microspores using centrifugation on 20% maltose was developed. The concentration of microspores was important and greatly increased the number of developing structures. Initial culture of microspores on FHG medium containing 62 g 1(-1) maltose, 4.4 mu M (1 mg 1(-1)) BA and 200 g 1(-1) Ficoll-400 resulted in high frequencies of plant regeneration. Albino plant frequency was correlated to length of time in culture. Stock plant condition appeared to be a major factor influencing induction frequency. From 668 to 1738 green plants per 100 anthers were produced. The number of calli and embryos obtained and the number of green plantlets regenerated were improved by increasing the Ficoll concentration from 100 g 1(-1) to 400 g 1(-1) during the culture period compared to continuous culture on FHG Ficoll 200 g 1(-1).

引用

页码：163 / 169

页数：7

共 26 条

[1] IDENTIFICATION OF EMBRYOGENIC MICROSPORES OF BARLEY (HORDEUM-VULGARE L) BY INDIVIDUAL SELECTION AND CULTURE AND THEIR POTENTIAL FOR TRANSFORMATION BY MICROINJECTION [J].