EFFICIENCY OF CHEMICAL OLIGONUCLEOTIDE SYNTHESIS EVALUATED BY ION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：9

作者：

FOLDESPAPP, Z

BIRCHHIRSCHFELD, E

ROSCH, R

HARTMANN, M

KLEINSCHMIDT, AK

SELIGER, H

机构：

[1] INST BIOTECHNOL EV,D-07745 JENA,GERMANY

[2] UNIV ULM,D-89069 ULM,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 706卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01221-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ion-exchange high-performance liquid chromatography (HPIEC) is a method of choice for the separation of crude solid-phase synthesized oligonucleotides. It shows peaks for target and error sequences and it is used for quantification of oligonucleotide synthesis. This paper presents a homeodynamic model of polymer-supported oligonucleotide synthesis and a numerical solution, which accounts for elution profiles obtained by HPIEC. The elution profiles are consistent with the model. The chain-length distributions for 30mer syntheses on different support materials were also studied and the probability moment analysis was applied as a quantitative measure of the differential relationship of fractal dimension. The support systems for chemical synthesis of short oligonucleotides are classified according to this relationship.

引用

页码：405 / 419

页数：15

共 39 条

[1]

AGRAWAL S, 1994, METHODS MOL BIOL, V26

[2]

BARNETT RW, 1990, NUCLEIC ACIDS RES, V18, P3098

[3]

BAUMANN G, 1994, FRACTALS IN BIOLOGY AND MEDICINE, P182

[4] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].

BEAUCAGE, SL ;

CARUTHERS, MH .

TETRAHEDRON LETTERS, 1981, 22 (20) :1859-1862

[5] A VERSATILE SUPPORT FOR THE SYNTHESIS OF OLIGONUCLEOTIDES OF EXTENDED LENGTH AND SCALE [J].

BIRCHHIRSCHFELD, E ;

FOLDESPAPP, Z ;

GUHRS, KH ;

SELIGER, H .

NUCLEIC ACIDS RESEARCH, 1994, 22 (09) :1760-1761

[6]

BIRCHHIRSCHFELD E, 1992, 3RD SWED GERM WORKSH, P35

[7]

Bunde A., 1994, FRACTALS SCI

[8]

CAMEL AB, 1993, APPLIED CHAOS THEORY

[9]

CARUTHERS MH, 1992, METHOD ENZYMOL, V211, P3

[10]

Casti J. L, 1992, REALITY RULES, VII

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