BICARBONATE STIMULATION OF NA+ TRANSPORT IN LIVER BASOLATERAL PLASMA-MEMBRANE VESICLES REQUIRES THE PRESENCE OF A TRANSMEMBRANE PH GRADIENT

被引：10

作者：

FELIPE, A ^{[1
]}

MOULE, SK ^{[1
]}

MCGIVAN, JD ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1029卷 / 01期

基金：

英国惠康基金;

关键词：

(Rat liver); Bicarbonate; pH gradient; Plasma membrane; Sodium ion transport;

D O I：

10.1016/0005-2736(90)90436-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of bicarbonate on the uptake of 22Na+ into liver basolateral plasma membrane vesicles was studied under conditions where the pH of the medium was controlled by the use of high buffer concentrations. Bicarbonate stimulated the rate of Na+ uptake only in the presence of a pH gradient (acid inside). The stimulation by bicarbonate was inhibited by both amiloride and DIDS. No evidence for electrogenic Na+/HCO3- symport was found. These results are in part consistent with electroneutral Na+/HCO3- symport, but other explanations cannot be excluded. © 1990.

引用

页码：61 / 66

页数：6

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