TYROSINE-7 IS AN ESSENTIAL RESIDUE FOR THE CATALYTIC ACTIVITY OF HUMAN CLASS PI GLUTATHIONE-S-TRANSFERASE - CHEMICAL MODIFICATION AND SITE-DIRECTED MUTAGENESIS STUDIES

被引：62

作者：

KONG, KH ^{[1
]}

NISHIDA, M ^{[1
]}

INOUE, H ^{[1
]}

TAKAHASHI, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91848-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glutathione (GSH)-conjugating activity of human class Pi glutathione S-transferase (GSTπ) toward 1-chloro-2,4-dinitrobenzene (CDNB) was significantly lowered by reaction with N-acetylimidazole, an O-acetylating reagent for tyrosine residues. Further, the replacement of Tyr7 in GSTπ, which is conserved in all cytosolic GSTs, with phenylalanine by sitedirected mutagenesis also lowered the activities toward CDNB and ethacrynic acid. The Km values of the mutant for both GSH and CDNB were almost equivalent to those of the wild type, while the Vmax of the former was about 55-fold smaller than that of the latter. Therefore, Tyr7 is considered to be an essential residue for the catalytic activity of GSTπ. © 1992.

引用

页码：1122 / 1129

页数：8

共 26 条

[1] CHEMICAL MODIFICATION OF RAT-LIVER MICROSOMAL GLUTATHIONE TRANSFERASE DEFINES RESIDUES OF IMPORTANCE FOR CATALYTIC FUNCTION [J].

ANDERSSON, C ;

MORGENSTERN, R .

BIOCHEMICAL JOURNAL, 1990, 272 (02) :479-484

[2] EVIDENCE FOR THE INVOLVEMENT OF HISTIDINE AT THE ACTIVE-SITE OF GLUTATHIONE-S-TRANSFERASE PHI FROM HUMAN-LIVER [J].

AWASTHI, YC ;

BHATNAGAR, A ;

SINGH, SV .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 143 (03) :965-970

[3] RELATIONSHIP BETWEEN SOLUBLE GLUTATHIONE-DEPENDENT DELA-5-3-KETOSTEROID ISOMERASE AND GLUTATHIONE S-TRANSFERASES OF LIVER [J].

BENSON, AM ;

TALALAY, P ;

KEEN, JH ;

JAKOBY, WB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (01) :158-162

[4] DISSECTION OF THE CATALYTIC MECHANISM OF ISOZYME 4-4 OF GLUTATHIONE S-TRANSFERASE WITH ALTERNATIVE SUBSTRATES [J].

CHEN, WJ ;

GRAMINSKI, GF ;

ARMSTRONG, RN .

BIOCHEMISTRY, 1988, 27 (02) :647-654

[5]

DESIDERI A, 1991, J BIOL CHEM, V266, P2063

[6]

KANO T, 1987, CANCER RES, V47, P5626

[7] CHEMICAL MODIFICATION OF TYROSINE RESIDUES IN RIBONUCLEASE-T1 WITH N-ACETYLIMIDAZOLE AND P-DIAZOBENZENESULFONIC ACID [J].

KASAI, H ;

TAKAHASHI, K ;

ANDO, T .

JOURNAL OF BIOCHEMISTRY, 1977, 81 (06) :1751-1758

[8] NON-ESSENTIALITY OF CYSTEINE AND HISTIDINE-RESIDUES FOR THE ACTIVITY OF HUMAN CLASS PI GLUTATHIONE-S-TRANSFERASE [J].

KONG, KH ;

INOUE, H ;

TAKAHASHI, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 181 (02) :748-755

[9] IDENTIFICATION OF A HIGHLY REACTIVE SULFHYDRYL-GROUP IN HUMAN PLACENTAL GLUTATHIONE TRANSFERASE BY A SITE-DIRECTED FLUORESCENT REAGENT [J].

LOBELLO, M ;

PETRUZZELLI, R ;

DESTEFANO, E ;

TENEDINI, C ;

BARRA, D ;

FEDERICI, G .

FEBS LETTERS, 1990, 263 (02) :389-391

[10] REACTION OF PURIFIED BOVINE THROMBIN WITH N-ACETYLIMIDAZOLE [J].

LUNDBLAD, RL ;

HARRISON, JH ;

MANN, KG .

BIOCHEMISTRY, 1973, 12 (03) :409-414

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